BisulFlash DNA Modification Kit from EpiGentek

Product Specs
ItemBisulFlash DNA Modification Kit
CompanyEpiGentek
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP-1026
Quantity50 Reactions
ApplicationsAmount Quantitation
FormatMicroplate-based
Sample TypeDNA
Research AreaDNA Methylation

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956

Email: info@epigentek.com
sales@epigentek.com
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support@epigentek.com

Company Profile

Website: www.epigentek.com

(1) Trained immunity of alveolar macrophages enhances injury resolution via KLF4-MERTK-mediated efferocytosisJ Exp MedNovember 6, 2023Sreeparna Chakraborty, Abhalaxmi Singh, Li Wang, Xinge Wang, Mark A. Sanborn, Zijing Ye, Mark Maienschein-Cline, Amitabha Mukhopadhyay, Balaji B. Ganesh, Asrar B. Malik, et al.manufacturer’s instructions. Extracted genomic DNA (500 ng) was treated with BisulFlash DNA Modification Kit (P-1026-050; Epigentek) according to the manufacturer’s protocol. 1 μl of 20 μl eluate was used for real time

(2) PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cellsJournal of experimental & clinical cancer research : CRNovember 8, 2021You JH, Lee J, Roh JL.Extracted genomic DNA was converted into a bisulfite form using a BisulFlash DNA Modification Kit (P-1026-050; EpiGentek, Farmingdale, NY, USA). The degree of methylation was determined in SIRT1 by RT-qPCR

(3) DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brainDevelopmental BiologyJune 1, 2020Yasuhiro Kyono, Samhitha Raj, Christopher J. Sifuentes, Nicolas Buisine, Laurent Sachs, Robert J. Denverv2 kit,"Illumina Inc., San Diego, CA",RS-122-2001 DNA modification kit,"Epigentek, Farmingdale, NY",P-1026-050 Deposited Data,Deposited Data,Deposited Data Raw and analyzed MethylCap-seq data,This paper,GSE139267 Raw

(4) Loss of Lkb1 impairs Treg function and stability to aggravate graft-versus-host disease after bone marrow transplantationCellular & Molecular ImmunologyMay 1, 2020Xiuhua Su et al.Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) and bisulfite-transformed with the EZ DNA Methylation Direct Kit (P-1026-050, Epigentek, Farmingdale, NY, USA) according to the manufacturers’ instructions. Specific polymerase chain reaction (PCR

(5) Canonical cytosolic iron-sulfur cluster assembly and non-canonical functions of DRE2 in ArabidopsisPLoS geneticsApril 1, 2019Wang X, Chen X, Sun L, Qian W.bisulfite sequencing About 100 ng of genomic DNA was modified using the BisulFlash DNA Modification Kit (Epigentek, P-1026-050) according to the manufacturer’s instructions. An aliquot (1 μL) of PLOS Genetics | https://doi.org/10.1371/journal.pgen.1008094

(6) Anaphase-promoting complex/cyclosome regulates RdDM activity by degrading DMS3 in ArabidopsisProceedings of the National Academy of SciencesFebruary 26, 2019Songxiao Zhong et al.Methods. DNA Methylation Assay. Genomic DNA was treated using the BisulFlash DNA Modification Kit (catalog no. P-1026-050; Epigentek). Details are provided in SI Appendix, Supplemental Materials and Methods. Synchronization

(7) Voltammetric Application of Polypyrrole-Modified Microelectrode Array for the Characterization of DNA Methylation in Glutathione S-Transferase Pi 1Analytical LettersNovember 2, 2018Kenton Meronard, Mira Josowicz, Amir Saheb(10):1192–99. 10.1038/nbt873. Epigentek. 2015 BisulFlash ™ DNA Modification Kit. https://www.epigentek.com/docs/P-1026.pdf. Frommer M, McDonald LE, Millar DS, Collis CM, Watt F, Grigg GW, Molloy PL, and Paul CL. 1992 A

(8) DNA methylation rather than single nucleotide polymorphisms regulates the production of an aberrant splice variant of IL6R in mastitic cowsCell Stress and ChaperonesJuly 1, 2018Yan Zhang et al.mastitisinfected cows was used for bisulfate modification with a BisulFlash DNA Modification Kit (Epigentek, Cat. #P1026) according to the manufacturer’s instructions. DNA was stored at − 20 °C until use as templates for

(9) Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCRPLOS ONEJune 14, 2018Sam Kint, Ward De Spiegelaere, Jonas De Kesel, Linos Vandekerckhove, Wim Van Criekingelow) qPCR dPCR Conversion (ranking) (ranking) efficiency (% ± SD) 1 Bisulflash™ DNA Modification Kit (Epigentek, P-1026) Bisulflash 95 20 350 9 - 11 10 Not Assayed 2 Bisulflash™ DNA Bisulflash Easy Bisulfite Conversion Easy

(10) The construction of intrahepatic cholangiocarcinoma model in zebrafishScientific ReportsDecember 1, 2017Jing Wang, Xiaoqian Leng, Guiping Wang, Xiaoyang Wan, Hong Caobisulfite to convert cytosine to uracil using the BisulFlash™ DNA Modification kit (Epigentek, USA, P-1026-050) according to the manufacturer’s instruction. Bisulfite sequencing was performed as described previously51,

(11) Lkb1 maintains Treg cell lineage identityNature CommunicationsAugust 1, 2017Di Wu et al.converted with EZ DNA Methylation-Direct kits according to the manufacturer’s protocol (Epigentek, P-1026-050). Methylation-specific PCR primers (listed in Supplementary Data 2) were used for amplification

(12) Mir-449a, a potential diagnostic biomarker for WNT group of medulloblastomaJournal of neuro-oncologySeptember 1, 2016Yongxiao Li, Tao Jiang, Liwei Shao, Yan Liu, Chen Zheng, Yanfeng Zhong, Jing Zhang, Qing Chang

(13) The cytosolic Fe-S cluster assembly component MET18 is required for the full enzymatic activity of ROS1 in active DNA demethylationScientific ReportsSeptember 1, 2016Xiaokang Wang, Qi Li, Wei Yuan, Zhendong Cao, Bei Qi, Suresh Kumar, Yan Li, Weiqiang Qian

(14) Global DNA Methylation Changes in Nile Tilapia Gonads during High Temperature-Induced MasculinizationPLOS ONEAugust 3, 2016Li-Xue Sun, Yi-Ya Wang, Yan Zhao, Hui Wang, Ning Li, Xiang Shan Ji

(15) Plasmodium yoelii infection of BALB/c mice results in expansion rather than induction of CD4(+) Foxp3(+) regulatory T cellsImmunologyJune 1, 2016Simone Abel, Kristina Ueffing, Roman Tatura, Marina Hutzler, Matthias Hose, Kai Matuschewski, Jan Kehrmann, Astrid M Westendorf, Jan Buer, Wiebke Hansen

(16) AP2α controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progressionOncogeneJune 1, 2016N Felli et al.

(17) Utility of Serum miR-125b as a Diagnostic and Prognostic Indicator and Its Alliance with a Panel of Tumor Suppressor Genes in Epithelial Ovarian CancerPLOS ONEApril 19, 2016Mariyam Zuberi, Imran Khan, Rashid Mir, Gauri Gandhi, Prakash Chandra Ray, Alpana Saxena

(18) Epigenetic modification of PKMζ rescues aging-related cognitive impairmentScientific reportsMarch 1, 2016Chen C, Meng SQ, Xue YX, Han Y, Sun CY, Deng JH, Chen N, Bao YP, Zhang FL, Cao LL, et al.

(19) Dicer-independent RNA-directed DNA methylation in ArabidopsisCell researchJanuary 1, 2016Dong-Lei Yang, Guiping Zhang, Kai Tang, Jingwen Li, Lan Yang, Huan Huang, Heng Zhang, Jian-Kang Zhu

(20) ATRX loss in adult supratentorial diffuse astrocytomas correlates with p53 over expression and IDH1 mutation and predicts better outcome in p53 accumulated patientsHistology and histopathologyJanuary 1, 2016Li-Wei Shao, Yi Pan, Xue-Ling Qi, Yong-Xiao Li, Xiao-Long Ma, Wei-Ning Yi, Jing Zhang, Yan-Feng Zhong, Qing Chang

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